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We report on changes in the Raman spectrum of live Escherichi coli (E.coli)that result from exposure to lethal fluences of 300 nm (UV-B) and 405 nm(violet) photons. In the first instance, the energy per photon of 4.13 eV issufficient to break most of the inter-atomic bonds in the bacterium and majorchange in the Raman spectrum, particularly in the RNA/DNA regions is observed.This energy is in near resonance with the C-H, N-H, and P-O bond bindingenergies that interconnect phosphate backbone sections and may initiate theRaman modifications. By contrast, the 3.06 eV violet photon energy isinsufficient to cleave the stronger system bonds. The much larger lethalfluence required in this case produces a significant change in the resonant C-Nand C-P bond connected amino acid/protein groups and lipid peak signal but muchless so in the nucleotide-DNA/RNA signature.

Confocal micro-Raman Spectra of untreated and lethally treated Escherichia coli exposed to UV-B and violet light