English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Heparin is an important anticoagulant drug, about one billion doses areproduced annually. It is a polydisperse sulfated polysaccharide, and theinherent heterogeneity makes the analysis of heparin difficult. The globalcrisis resulting from adulterated heparin in 2008 has drawn renewed attentionto the challenges that are associated with the quality control andcharacterization of this complex biological medicine from natural sources. Thepresent study addresses the need for simple and user-friendly analyticalmethods for the fast and accurate quantification of heparin in complexmatrices. Direct quantification of heparin in the low microgram per mL rangewas accomplished using a specific commercially available assay based on thefluorescent molecular probe Heparin Red, simply by mixing the heparincontaining sample and a reagent solution in a 96-well microplate followed byfluorescence readout. A screening of typical impurities in raw heparin(selected other glycosaminoglycans, residual nucleic acids and proteins),related to the extraction from animal tissues, as well as of components of theurine matrix (inorganic salts, amino acids, trace proteins) revealed that thesecompounds even in large excess have no or very little effect on the accuracy ofheparin determination. Heparin spike detection in urine, a biologicalmulticomponent matrix, also showed good accuracy. We envision applications ofthis mix-and-read assay in the process and quality control in heparinmanufacturing, but also in pharmacokinetic studies as a convenient tool formeasuring of the urinary excretion of heparins.

Quantification of heparin in complex matrices (including urine) using a mix-and-read fluorescence assay