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Tuna are large pelagic fish whose populations are close to panmixia. Inaddition, they are threatened species, so it is important for the maintenanceand monitoring of genetic diversity that genetic information at a genome levelbe obtained. Here we report the draft assembly of the southern bluefin tunagenome and the collection of genome-wide sequence data for five other tunaspecies. We sampled five tuna species of the genus Thunnus, the northern andsouthern bluefin, yellowfin, albacore, and bigeye, as well as the skipjack(Katsuwonis pelamis), a tuna-like species. Genome assembly was facilitated atk-mer=25 while k-mer=51 generated assembly artefacts. The estimated size of thesouthern bluefin tuna genome was 795 Mb. We assembled two southern bluefin tunaindividuals independently using both paired end and mate pair sequence. Thisresulted in scaffolds with N50>174,000 bp and maximum scaffold lengths>1.4 Mb.Our estimate of the size of the assembled genome was the scaffolded sequencesin common to both assemblies, which amounted to 721 Mb of the 795 Mb of thesouthern bluefin tuna genome sequence. Using BLAST, there were matches between13,039 of 14,341 (91%) refseq mRNA of the zebrafish Danio rerio to the tunaassembly indicating that most of a generic fish transcriptome was covered bythe assembly.

A draft genome assembly of southern bluefin tuna Thunnus maccoyii