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Intravital microscopy is an essential tool that reveals behaviours of livecells under conditions close to natural physiological states. So far, althoughvarious approaches for imaging cells in vivo have been proposed, most requirethe use of labelling and also provide only qualitative imaging information.Holographic imaging approach based on measuring the refractive indexdistributions of cells, however, circumvent these problems and offerquantitative and label-free imaging capability. Here, we demonstrate in vivotwo- and three-dimensional holographic imaging of circulating blood cells inintact microcapillaries of live mice. The measured refractive indexdistributions of blood cells provide morphological and biochemical propertiesincluding three-dimensional cell shape, haemoglobin concentration, andhaemoglobin contents at the individual cell level. With the present method,alterations in blood flow dynamics in live healthy and sepsis-model mouse werealso investigated.

Holographic intravital microscopy for 2-D and 3-D imaging intact circulating blood cells in microcapillaries of live mice