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Measuring microorganisms in food products is a critical issue for food safetyand human health. Although various approaches for detecting low-levels ofmicroorganisms in food have been developed, they require high-cost, complexequipment, invasive procedures, and skilled technicians which limit theirwidespread use in the food industry. Here, we present a simple,non-destructive, non-contact, and rapid optical method for measuring livingmicroorganisms in meat products using laser speckle decorrelation. By simplymeasuring dynamic speckle intensity patterns reflected from samples andanalyzing the temporal correlation time, the presence of living microorganismscan be non-invasively detected with high sensitivity. We presentproof-of-principle demonstrations for detecting E. coli and B. cereus inchicken breast tissues.

A simple and rapid method for detecting living microorganisms in food using laser speckle decorrelation