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One of the most challenging tasks in microarray image analysis is spotsegmentation. A solution to this problem is to provide an algorithm than can beused to find any spot within the microarray image. Circular HoughTransformation (CHT) is a powerful feature extraction technique used in imageanalysis, computer vision, and digital image processing. CHT algorithm isapplied on the cDNA microarray images to develop the accuracy and theefficiency of the spots localization, addressing and segmentation process. Thepurpose of the applied technique is to find imperfect instances of spots withina certain class of circles by applying a voting procedure on the cDNAmicroarray images for spots localization, addressing and characterizing thepixels of each spot into foreground pixels and background simultaneously.Intensive experiments on the University of North Carolina (UNC) microarraydatabase indicate that the proposed method is superior to the K-means methodand the Support vector machine (SVM). Keywords: Hough circle transformation,cDNA microarray image analysis, cDNA microarray image segmentation, spotslocalization and addressing, spots segmentation

Robust cDNA microarray image segmentation and analysis technique based on Hough circle transform