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The selective insertion of foreign materials, such as fluorescent markers orplasmids, into living cells has been a challenging problem in cellbiology.However, it is often necessary that researchers insert such materialsinto cells for various dynamical and/or drug delivery studies. This problembecomes even more challenging if the study is to be limited to specific cellswithin a larger population, since other transfection methods, such as viraltransfection and lipofection, are not realizable with a high degree of spatialselectivity.Here, we have used a focused femtosecond laser beam to create asmall transient hole in the cellular membrane (optoporation) in order to injectnanomolar concentrations of rhodamine phalloidin (an impermeable dye moleculefor staining filamentous actin) into targeted living mammalian cells (both HEKand primary cortical neurons).Following optoporation, the bound to theintracellular actin network and rise in fluorescence intensity was observed...

Introduction of impermeable actin-staining molecules to mammalian cells by optoporation