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Development of reliable and eco-friendly processes for synthesis of metallicnanoparticles is an important step in the field of application ofnanotechnology. In the present study, extracellular synthesis of silvernanoparticles using culture supernatant of plant growth promotingrhizobacterium, Pseudomonas sp.ARS22. The biosynthesis of AgNPs by Pseudomonassp.ARS22 was monitored by UVvisible spectrum that showed the surface plasmonresonance peak at 410 nm, an important characteristic of AgNPs. Furthermore,the morphological, elemental, functional and thermal characterization ofarsAgNPs was carried out using the electron and atomic microscopies, FTIRspectroscopy and Atomic Force microscopy, respectively. The arsAgNPs werespherical in shape. The DebyeScherrer equation was used to calculate particlesizes and the silver nanoparticles of approximate size 10 to 30 nm wereobserved. The process of reduction is extracellular, which makes it an easiermethod for the synthesis of silver nanoparticles.

Extracellular biosynthesis of silver nanoparticles from Plant Growth Promoting Rhizobacteria Pseudomonas sp