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Adaptive optics is a strategy to compensate for sample-induced aberrations inmicroscopy applications. Generally, it requires the presence of "guide stars"in the sample to serve as localized reference targets. We describe animplementation of conjugate adaptive optics that is amenable to widefield (i.e.non-scanning) microscopy, and can provide aberration corrections overpotentially large fields of view without the use of guide stars. A uniquefeature of our implementation is that it is based on wavefront sensing with asingle-shot partitioned-aperture sensor that provides large dynamic rangecompatible with extended samples. Combined information provided by this sensorand the imaging camera enable robust image de-blurring based on a rapidestimation of sample and aberrations obtained by closed-loop feedback. Wepresent the theoretical principle of our technique and proof of conceptexperimental demonstrations.

Conjugate adaptive optics in widefield microscopy with an extended-source wavefront sensor