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We present a graphene/Cu nanoparticle hybrids (G/CuNPs) system as asurface-enhanced Raman scattering (SERS) substrate for adenosine detection. TheCu nanoparticles wrapped around a monolayer graphene shell were directlysynthesized on flat quartz by chemical vapor deposition in a mixture of methaneand hydrogen. The G/CuNPs showed an excellent SERS enhancement activity foradenosine. The minimum detected concentration of the adenosine in serum wasdemonstrated as low as 5 nM, and the calibration curve showed a good linearresponse from 5 to 500 nM. The capability of SERS detection of adenosine inreal normal human urine samples based on G/CuNPs was also investigated and thecharacteristic peaks of adenosine were still recognizable. The reproducible andthe ultrasensitive enhanced Raman signals could be due to the presence of anultrathin graphene layer. The graphene shell was able to enrich and fix theadenosine molecules, which could also efficiently maintain chemical and opticalstability of G/CuNPs. Based on G/CuNPs system, the ultrasensitive SERSdetection of adenosine in varied matrices was expected for the practicalapplications of medicine and biotechnology

Direct Growth Graphene on Cu Nanoparticles by Chemical Vapor Deposition  as Surface-Enhanced Raman Scattering Substrate for Label-Free Detection of  Adenosine