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Rhizoctonia solani anastomosis group AG2-2 IIIB is a severe sugar beet andmaize pathogen. It causes crown and root rot disease which leads to yieldlosses world-wide. The soil-borne pathogen is difficult to detect and quantifyby conventional methods. We developed a real-time PCR (qPCR) assay for thequantification of genomic DNA of Rhizoctonia solani AG2-2 IIIB based on the ITSregion of rDNA genes. The limit of quantification of the assay is 1.8 pggenomic DNA. The amplification efficiency was 96.4. The assay will be helpfulin the diagnoses of Rhizoctonia solani infection of sugar beet and maize rootsand in the quantification of R. solani AG2-2 IIIB inoculum in plant debris andsoil.

Real-time PCR (qPCR) assay for Rhizoctonia solani anastomoses group AG2-2IIIb