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We demonstrate that endonuclease deficient Clustered Regularly InterspacedShort Palindromic Repeats CRISPR-associated Cas9 protein (dCas9) fused to thephoto-convertible fluorescence protein monomeric mEos3.1 (dCas9-mEos3) can beused to resolve sub-diffraction limited features of repetitive gene elements,thus providing a new route to investigate high-order chromatin organization atthese sites.

CRISPR/Cas9 For Photoactivated Localization Microscopy (PALM)