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Binding of acridine derivative quinacrine (QA) to chicken erythrocyte DNA wasstudied by methods of absorption and polarized fluorescent spectroscopy.Measurements were carried out in aqueous buffered solutions (pH 6.9) ofdifferent dye concentrations (QA concentration range from $10^{-6}$ till$10^{-4}$ M) and ionic strengths ($Na^{+}$ concentration rang from $10^{-3}$till 0.15 M) in a wide range of phosphate-to-dye molar ratios ($P/D$). It isestablished that the minimum of fluorescent titration curve plotted as relativefluorescence intensity $vs$ $P/D$ is conditioned by the competition between thetwo types of QA binding to DNA which posses by different emission parameters:(i) intercalative one dominating under high $P/D$ values, and (ii) outsideelectrostatic binding dominating under low $P/D$ values, which is accompaniedby the formation of non-fluorescent dye associates on the DNA backbone.Absorption and fluorescent characteristics of complexes formed were determined.The method of calculation of different binding modes contribution to thecomplex formation depending on $P/D$ value is presented. It was shown that thesize of binding site measured as the number of DNA base pairs per one QAmolecule bound in the case of the electrostatic interaction is 8 times lessthan that for the intercalative one that determines the competitive ability ofthe outside binding against the stronger intercalative binding mode.

Calculating the contribution of different binding modes to Quinacrine - DNA complex formation from polarized fluorescence data