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Real time laser induced fluorescence spectroscopy usage for microanalysis ofDNA double helix defects is shown. The method is based on Forster resonanceenergy transfer (FRET) in intercalator-donor pair (acridine orange as a donorand ethidium bromide as an acceptor). Transition metal ions such as Cu(II),Cu(I), Ag(I), silver nanoparticles (AgNPs), photo- and thermo effects were usedto cause double helix defects in DNA.  FRET radii were experimentally estimated in background electrolyte solution(0.01 M NaNO3) and proved to be 3.9 +- 0.3 nm and the data are in satisfactoryagreement with the theoretically calculated value Ro = 3.5 +- 0.3 nm.  Concentration of DNA sites, exposed to Cu(II), Cu(I), Ag(I) ions, AgNPsimpact as well as laser irradiation ({\lambda} = 457 nm) and temperature, whichare applicable for intercalation, were estimated in relative units.  FRET method allows to estimate the concentration of double helix areas withhigh quality stability applicable for intercalation in DNA after it wassubjected to stress effect. It gives the opportunity to compare DNAs of 1)different origin; 2) with various damage degrees; 3) being in variousfunctional state.

Forster Resonance Energy Transfer and Laser Fluorescent Analysis of Defects in DNA Double Helix