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In this manuscript we present an in vitro model based on agarose gel that canbe used to simulate a dirty, oily, bloody, and morphologically complex surfaceof, for example, an open wound. We show this models effectiveness in simulatingdepth of penetration of reactive species generated in plasma deep into tissueof a rat and confirm the penetration depths with agarose gel model. We envisionthat in the future such a model could be used to study plasma discharges (andother modalities) and minimize the use of live animals: plasma can be optimizedon the agarose gel wound model and then finally verified using an actual wound.

Penetration Deep into Tissues of Reactive Oxygen Species Generated in  Floating-Electrode Dielectric Barrier Discharge (FE-DBD): in Vitro Agarose  Gel Model Mimicking an Open Wound