English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Single-molecule sensitive microscopies and spectroscopies are transformingbiophysics and materials science laboratories. Techniques such as fluorescencecorrelation spectroscopy (FCS) and single-molecule sensitive fluorescenceresonance energy transfer (FRET) are now commonly available in researchlaboratories but are as yet infrequently available in teaching laboratories. Wedescribe inexpensive electronics and open-source software that bridges thisgap, making state-of-the-art measurement research capabilities accessible toundergraduates interested in biophysics. We include a pedagogical discussion ofthe intensity correlation function relevant to FCS and its calculation directlyfrom photon arrival times. We demonstrate the system with a measurement of thehydrodynamic radius of a protein using FCS that is suitable for anundergraduate teaching laboratory. The FPGA-based electronics, which are easyto construct, are suitable for more advanced measurements as well, and severalapplications are demonstrated. As implemented, the system has 8 ns timingresolution, outputs to control up to four laser sources, and inputs for as manyas four photon-counting detectors.

Inexpensive hardware and software for photon statistics and correlation spectroscopy