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Existing methods of heart rate recording in animals have shortcomings, whichsignificantly obscure the influence of experimental factors on heart rate. Wehave developed a method of electrocardiographic recording of heart rate in ratswithout these drawbacks. To contact the animal limbs used liquid electrodeswhich are two small baths filled with conductive fluid (saline solution). Toprovide the relative immobility (and quiet) of the animal the two baths wascovered with a dark chamber without a bottom and with an entrance for the rat.During the experiment, a rat placed near the chamber comes into it (for theinnate preference for darkness) and locates itself inside with its head for theexit. At that moment ECG recording starts. This method allows to record heartrate in the intact rodents (without anesthesia and stress) and does not requiresubstantial preparation. It is not suitable for standard ECG analysis of theheart condition and function, but this is a good way for recording heart ratefor the further analysis of its variability.

Noninvasive method for electrocardiogram recording in conscious rodents with the electro-conductive liquid electrodes