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Fluorescence correlation and cross-correlation spectroscopy (FCS, FCCS) arewidely used techniques to study the diffusion properties and interactions offluorescent molecules. Autocorrelation (ACFs) and cross-correlation functions(CCFs) are typically acquired with fast hardware correlators. Here we introducea new multiple-{\tau} hardware correlator design for computing ACFs and CCFs inreal time. A scheduling algorithm minimizes the use of hardware resources bycalculating the different segments of the correlation function on a singlecorrelator block. The program was written in LabVIEW, enabling computation oftwo multiple-{\tau} ACFs and two CCFs on a National Instruments FPGA card (NI7833R) in real time with a minimal sampling time of 400 ns. Raw data are alsostored with a time resolution of 50 ns for later analysis. The design can beadapted to other FPGA cards with only minor changes and extended to evaluatemore inputs and correlation functions.

Multiplexed multiple-{\tau} auto- and cross- correlators on a single FPGA