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We have measured the fluorescence spectrum for fluorescein solution inethanol with concentration 1 {\times} 10-3 mol/liter at different temperaturesfrom room temperature to freezing point of solvent, (T = 153, 183, 223, 253,and 303 K) using liquid nitrogen. Table curve 2D version 5.01 program has beenused to determine the fitting curve and fitting equation for each fluorescencespectrum. Fourier series (3 {\times} 2) was the most suitable fitting equationfor all spectra. Theoretical fluorescence spectrum of fluorescein in ethanol atT = 183K was calculated and compared with experimental fluorescence spectrum atthe same temperature. There is a good similarity between them.

Fluorescence spectrum analysis using Fourier series modeling for Fluorescein solution in Ethanol