English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Engineered inorganic nanoparticles are essential components in thedevelopment of nanotechnologies. For applications in nanomedicine, particlesneed to be functionalized to ensure a good dispersibility in biological fluids.In many cases however, functionalization is not sufficient : the particlesbecome either coated by a corona of serum proteins or precipitate out of thesolvent. In the present paper, we show that by changing the coating of ironoxide nanoparticles from a low-molecular weight ligand (citrate ions) to smallcarboxylated polymers (poly(acrylic acid)), the colloidal stability of thedispersion is improved and the adsorption/internalization of iron towardsliving mammalian cells is profoundly affected. Citrate-coated particles areshown to destabilize in all fetal-calf-serum based physiological conditionstested, whereas the polymer coated particles exhibit an outstandingdispersibility as well as a structure devoid of protein corona. Theinteractions between nanoparticles and human lymphoblastoid cells areinvestigated by transmission electron microscopy and flow cytometry. Two typesof nanoparticle/cell interactions are underlined. Iron oxides are found eitheradsorbed on the cellular membranes, or internalized into membrane-boundendocytosis compartments. For the precipitating citrate-coated particles, thekinetics of interactions reveal a massive and rapid adsorption of iron oxide onthe cell surfaces. The quantification of the partition between adsorbed andinternalized iron was performed from the cytometry data. The results highlightthe importance of resilient adsorbed nanomaterials at the cytoplasmic membrane.

The effects of aggregation and protein corona on the cellular internalization of iron oxide nanoparticles