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Technique of the quantitative description of image broadening effect in AFMallowing to restore real geometrical parameters of object using two-parametersmodel (height and width at half-height) is developed. Application of thetechnique has allowed to receive the quantitative information about molecularstructure of DNA-surfactant complexes.

Quantitative methods for deconvolution of true topographical properties of object on the basis of measured AFM-images: Part 2. Broadening effect