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Single strand breaks are induced in DNA plasmids, pBR322 and pUC19, inaqueous media by intense ultrashort laser pulses (820 nm wavelength, 45 fspulse duration, 1 kHz repetition rate) at intensities of 1-12 TW cm$^{-2}$. Theintense laser radiation generates, {\it in situ}, electrons that inducetransformation of supercoiled DNA into relaxed DNA. The extent ofelectron-mediated relaxation of DNA structure is quantified. Introduction ofelectron and radical scavengers inhibits DNA damage.

Effect of intense, ultrashort laser pulses on DNA plasmids in their native state: strand breakages induced by {\it in-situ} electrons