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A new strategy with bone tissue engineering by mesenchymal stem celltransplantation on titanium implant has been dawn attention. The surfacescaffold properties of titanium surface play an important role in boneregenerative potential of cells. The surface topography and chemistry arepostulated to be two major factors increasing the scaffold properties oftitanium implants. This study aimed to evaluate the osteogenic gene expressionof mesenchymal stem cells on titanium processed by wire-type electric dischargemachining. Some amount of roughness and distinctive irregular features wereobserved on titanium processed by wire-type electric discharge machining. Thethickness of suboxide layer was concomitantly grown during the processing.Since the thickness of oxide film and micro-topography allowed an improvementof mRNA expression of cells, titanium processed by wire-type electric dischargemachining is a promising candidate for mesenchymal stem cell based functionalrestoration of implants.

Bone regenerative potential of mesenchymal stem cells on a micro-structured titanium processed by wire-type electric discharge machining