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This study aimed to investigate the concentration of vitamin C in Momordica charantia (MC) by cultivar, harvest time, and maturity. The methods for determining vitamin C levels were validated by measuring their linearity, specificity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy using HPLC. Results showed high linearity in the calibration curve, with a coefficient of correlation (R) of 0.9994. The LOD and LOQ values for vitamin C were 0.05 and 0.16 μg/mL, respectively. The relative standard deviations (RSDs) for intraand inter-day precision of vitamin C measurements were 2.34 and 1.34%, respectively. Depending on cultivar, the concentration of vitamin C in MC varied from 20.75~107.31 mg/100 g, fresh weight, with an average level 68.85±25.57 mg/100 g, FW. When MC was analyzed by harvest time, the 20150612 MC showed the highest amount of vitamin C (113.20±1.89 mg/100 g, FW). On the other hand, the highest vitamin C content by maturity was 48.59±0.87 mg/100 g, FW (15 day old MC). This information on the comparative vitamin C levels of MC might be useful to food scientists and should be explored for functional food development.

Vitamin C Quantification of Korean Momordica charantia by Cultivar, Harvest Time, and Maturity