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The procedure described by Adebayo et al., (2010b) was adopted. Fresh leave of Chrysophyllum albidum was rinsed with distilled water to remove dust and other contaminant then air-dried in the laboratory for 14 days. The dried leave was subsequently mashed into fine powder using morta and pestle. The powder was weighed on weighing balance and recorded. 200g of C. albidum powder was weighed and transfer into conical flask of 1000ml. 250cm 3 of methanol was added to the measured powder in the conical flask gently swirled and placed on the table for 5 days. This was later filtered using Muslim clothes and cotton wool in a clean conical flask. The residue was weighed and recorded as well as the substrate. The substrate i.e. the extract was then evaporated using rotary evaporator at 50 o C. The extract was further evaporated with the use of water bath at 48 o C. The resultant extract was weighed and recorded accordingly. The same procedure was repeated using distilled water only that the substrate was not evaporated neither by rotary evaporator nor by water bath. A serial dilution of the two extracts was carried out in separate test-tubes. Results of antimicrobial activity show the activities of the plant extract as a broad spectrum since the Gram - positive and Gram - negative bacteria tested was sensitive to the extracts. The zones of inhibition obtained in respect of the extracts showed that organisms resistant to Ciprofloxacin were susceptible to the extracts. The methanol extracts had highest antimicrobial activities than that of water extracts. The methanol extract have pronounced activity on Candida albicans whereas it did not have any effect on Salmonella species. Key: MIC: Minimum Inhibitory Concentration, MBC: Minimum Bactericidal Concentration, R: Resistant, (+): Sensitive/ Presence (++): More Sensitive (+++): Most Sensitive, (-): Absent

iosr journal of applied chemistry

Phytochemical Analysis and Antimicrobial Effect of Chrysophillum Albidum Leave Extract On Gastrointestinal Tract Pathogenic Bacteria and Fungi in Human