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Rift valley fever virus (RVFV) causes severe disease, abortion and deaths in domestic animals (especially) young sheep, cattle and goats humans are infected by this virus through mosquitoes which maintain epizootic transmission being in contact with infected animal tissue. For this purpose, RVFV activities in Vero cell line media were exposed to different frequencies of amplitude modulated (AM) waves in the frequency range ( from 1 Hz to 10 Hz) for a period of 30 minutes /day along four successive days no measurable effect of the square amplitude modulated waves (QAMW) was noticed after the experiment in the Vero-cells. In another experiment 48 female Bulb/C mice were equally divided into 6 groups namely A1, A2, A3, A4, A5 & negative control.A1, A4 & A5 animals were infected by injection intraperitoneal (i.p) with dose of RVFV (0.15 mg/ Kg body weight (b.w) . A4 &A5 were exposed for 30 minutes /day along four successive days to 4.4 Hz & 5.2 Hz respectively while A1 without treatment any further treatment .A2 & A3 animals were exposed to 4.4 Hz & 5.2 Hz respectively for 30 minutes /day along four successive days, control group were injected 100 µl of saline by (i.p). At day 28 post injection of the animal, blood was collected for each group then after at sacrifice the liver enzymes (SGPT&SGOT),cellular changes were evaluated using cytokines expression (IFN- γ & IL-5) the exposed and non exposed ELF were studied survival rate and histopathology were demonstrated. The results: In vitro the data highly significant growth enhancement & inhibition respectively occurred in RVFV injected with vero cells after exposure to 4.4 Hz& 5.2 Hz respectively QAMW for 30 minutes/day along four successive days. In vivo the groups A1, A2&A3 highly significant increase in the serum level of SGPT&SGOT by less than A4&A5 groups respectively .The groups A2 &A3 were a significant increase in the serum level of IFN-γ. While, the serum level of IFN-γ of groups A1, A4 and A5 exhibited a significant decrease .Where the results were slightly increase but non-significant in level of IL-5 production in serum in groups A2 and A3. While the level of IL-5 of group A1 were a highly significant suppression more than in the groups A4 &A5 when compared to control. Histological sections of the exposed and non exposed were studied and survival rate were demonstrated.

Control the activity of Rift Valley Fever Virus by Electric Field waves at resonance frequency (In vivo &In vitro) studies