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The prevalence of fungal infections has raised in recent years due to an increasing population of immunosuppressed patients, intensive immunosuppressive chemotherapy, increasing awareness of fungal infections, and the widespread use of broad-spectrum antibiotics .Therefore, in the last few years, the development of novel diagnostic methods has been regarded a critical issue. A multiplex PCR strategy allowed the identification of 8 clinically relevant yeasts of the Candida genus, namely C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, C. guilliermondii, C. lusitaniae and C. dubliniensis. This method is relied on the amplification of two fragments from the ITS1 and ITS2 regions by the combination of 2 yeast-specific and 8 species specific primers in a single PCR reaction. This novel method provides a clinical diagnostic procedure with direct applicability.In addition to, it allows specific differentiation of individual Candida species within polyfungal samples.

Multiplex PCR identification of eight clinically relevant Candida species isolated from groin Fungal Infections among Egyptian patients