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On the basis of flower colour, the Apocynacean member Thevetia neriifolia,Juss. can be grouped into three morphoforms, viz. yellow, orange and white. Preliminary studies of different fractions of these three flower extracts (Petroleum ether (PE), Chloroform (CH), Ethyl Acetate (EA) and Methanol (MT) were carried out using Agar well diffusion method to evaluate the in vitro efficacy of antimicrobial properties against different pathogenic strains (Staphylococcus aureus, Streptococcus pyogenes, Nocardia asteroids, Pseudomonas aeruginosa, Proteus mirabilis, Candida albicans and Trichophyton rubrum) that cause human skin infections. Assays were carried out using different concentrations (10-1.25mg/50μl/6mm well) of the fractions and all organisms responded well in a dose dependent manner. Among the fractions, EA fraction showed maximum activity against N. asteroids (24.00±0.00 25.33±0.58mm) followed by P. mirabilis (21.33±1.15 22.33±1.15mm) and P. aeruginosa (19.67±1.53 21.00±1.41mm). MT fraction responded much better than CH fraction. PE fraction showed least activity against most of the tested pathogens. Assessment of antioxidant potential was done using DPPH free radical assay. EA fraction recorded a higher percentage of antioxidants with IC50 values 0.11-0.32mg/ml, followed by MT (0.3 0.45mg/ml) and CH (0.47 -0.9 mg/ml) fractions. Results of the present investigation revealed that flower extracts can be used as a good source of antimicrobial agent and as a natural antioxidant, justified the folkloric use of this medicinal plant.

Assessment of In vitro Antimicrobial and Antioxidant Potential of Thevetia neriifolia, Juss. Flower Morphoforms