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Seed samples were collected from 25 places of north eastern U.P from luxuriantly growing and healthy D.sissoo Roxb plants and stored at room temperature for 6 months.Mycofloral analysis through blotter method revealed the presence of 15 fungal species and agar plate method using czapeks dox agar medium showed the presence of 12 fungal species.During observation one insect-Bruchus pisorum was found to be present in all collected samples.The pathogenicity test put A.niger and Fusarium solani and insect-Bruchus pisorum highly potent and caused high degree of inhibition in germination and also caused high degree of mortality.Volatile constituents extracted in the form of essential oils from 32 plant species were evaluated against the dominant fungi Aspergillus niger and Fusarium solani as well as against insect-Bruchus bisporum.The seed kernel oil of Putranjiva exhibited the greatest toxicity.The oil was found to be fungicidal and thermostable at its minimum inhibitory concentration(MIC)400ppm.The oil was characterized by the determination of its various physicochemical properties. The oil protected the sissoo seeds completely for 6 months at 0.25ml (1000ppm)and 0.38 ml(1500ppm) in container of 250ml capacity holding 200g seeds.It did not exhibit any adverse effect on seed germination,seedling growth and general health and morphology of plants.Thus the seed kernel oil of Putranjiva showed potential as a preservative for sissoo seeds against spoilage by fungi and insects during storage.

Fumigant Potential of seed kernel oil of Putranjiva roxburghii Wall against storage pests of seeds of Dalbergia sissoo Roxb