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In order to study its development in-vitro, an investigation was carried out to determine and establish a suitable media for the growth of Ipomoea palmata Forssk, (Ipomoea. cairica). Aerial parts of a healthy plant were chosen as explants. Callogenesis of these leaf, stem and flower explants was initiated on three different media namely Murashige and Skoog (1962) media, Gamborg or B5 (1968) media and White's media, (1963). These basal media were supplemented with a quantity of coconut water and with various combinations of growth hormones- 2,4-Dichlorophenoxyacetic acid (2,4- D), Indole Acetic acid (IAA), Indole Butyric Acid ( IBA) and 6-Benzyl amino purine (BA). The observation showed that the explants regenerated into profuse calli which eventually proliferated into multiple shoots. Root formation from the callus was also observed. The highest response in callogenesis and morphogenesis was observed in M.S basal media supplemented with 4mg/l BA and 1mg/l 2,4-D. However development was first noticed in B5 basal media supplemented with 4mg/l and 1mg/l 2,4-D. I. palmata responded positively recording rapid growth, thereby, demonstrating both Re and De differentiation. Among the three different media, the low-salt media i.e. White's media showed a relatively poor response in callus and plantlet formation. Interestingly I.palmata seemed to have a great affinity for both MS and B5 media specifically, the hormonal concentration mentioned above. This report of a positive in-vitro response of I.palmata has not been published before.

An ideal media for the in-vitro propagation of Ipomoea palmata Forssk. [Synonym – Ipomoea cairica. L. Sweet] Convolulaceae