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The purpose of this study was to determine the prevalence of recurrent furunculosis in terms of age, gender and genetic basis from infected volunteers in university college hospital Ibadan Nigeria. A total of 102 isolates of Staphylococcus aureus from various anatomical pathological skin lesion were obtained from four hundred biochemically characterized samples. Antibiotic susceptibility profiles and minimum inhibitory concentration of the isolates was determine by Kirby Bauer and broth dilution method. β-lactamase potential of the isolates was determined by iodometric cell suspension methods and plasmid profiles by the use of lystostaphin for lyzing the cell wall. The percentage gender distributions were 46.0% females and 54.0% males. The isolates exhibited the lowest resistance of 11.75% to amoxicillin-clavulanic acid and 55.88% as the highest resistance to tetracycline. Thirty of the isolates possessed β-lactamase in varying degrees out of which 29 were plasmid-borne and 7.0 had multiple plasmid DNA of 2-4 copies, ranging between 2.20 and 23.10 kb. The resistance elicited by the strains evident in the Minimum Inhibitory Concentrations of the selected antibiotics and the associated R-plasmid encoded β-lactamase recorded accounted for the recurrent furunculosis which was found to vary among gender and age groups.

iosr journal of pharmacy and biological sciences

The Prevalence and Plasmid DNA Mediated Antibiotic Resistance of Isolates of Staphylococcus aureus From Recurrent Furunculosis in University College Hospital, Ibadan Nigeria.