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Fluorescence technique involves the optical detection and spectral analysis of light emitted by a substance undergoing a transition from an excited electronic state to a lower electronic state. The aim of this study is to assess the -amino levulinic acid (-ALA) uptake. Based on image processing technique, Matlab was used to analyze the fluorescence images resulted from activation of (-ALA) and follow its uptake along one week. Analyzing the RGB colours pixel profile from obtained results showed different profiles for malignant tissues, normal tissues, treated just after PDT and finally at one week post PDT. The treated tissues fluorescence profile showed changes from closer to malignant tissue profile till been closed to normal one. Conclusion: The results indicate that ALA phpotosensitizer tissue uptake can be identified using image analysis and processing for tissue f luorescence images by MATLAB.

Spatial Laser Induced Fluorescence Imaging (SLIFIMG) Analysis to Assess Tissue Photosensitizer Uptake