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In this study the production of substitute rennet by a newly isolated thermophilic fungus, Rhizomucor nainitalensis, using optimized solid substrate fermentation system have been explored. The results revealed that enzyme activity is at its highest level at 40˚C, 50 % humidity, inoculation volume of 10 3 spore /gr wheat bran, and exoenzyme biosynthesis induction by casein at a rate of 1.5 %. Enzyme activity reached its highest (8400 SU/gr wheat bran) after 110 hours of incubation under the stated conditions. Precipitated enzyme, by addition of ammonium sulfate with milk coagulating activity of 115000 SU/gr enzyme powder, was free of toxin and microorganisms, with market's favorable aroma. Keywords- Rhizomucor, Solid Substrate Fermentation, rennin, fungal proteases

Optimization of Fungal Rennet Production by local isolate of Rhizomucor nainitalensis Under Solid Substrate Fermentation system