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The objective of this work was to develop and validate simple, rapid, and accurate UV specrophotometric method for simultaneous determination of Ondansetron and Pantoprazole in enteric coated tablet dosage form. UV spectrophotometric method involves first derivative spectroscopy using 288.5 nm and 310 nm as zero crossing point for Pantoprazole and Ondansetron respectively. For spectrophotometric method, water was used as a solvent. Both the drug and their mix obeyed Beer- Lamberts law at selected wavelength was observed in concentration range of 0.5-2.5ug/ml of Ondansetron and 5-25ug/ml of Pantoprazole. Results of analysis were analyzed and validated for various parameters according to ICH guidelines. This method was found to be simple, precise, accurate, selective and rapid and can be successfully applied for the determination of pure laboratory mixtures and tablet formulations.

Simultaneous Estimation of Ondansetron and Pantoprazole in Solid Dosage Form by First Derivative Spectroscopy Method