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A successful protocol was developed for rapid clonal propagation of Tinospora formanii, a rare endemic medicinal plant of the family menispermaceae through the culture of mature nodal explants. Explants were disinfested with the use of 0.1% mercuric chloride and washed thoroughly with autoclaved distilled water. Solid MS medium was used with addition of different concentrations of 6-benzyl aminopurine (BAP) and indole butyric acid (IBA). The nodal segments inoculated in MS basal medium supplemented with BAP 2.0 mg L -1 and IBA0.5 mg L -1 initiated 2-3 buds in 6-7 days of culture. 95% of explants initiated shoots in this culture. Newly formed shoot clumps cultured on fresh medium with the same concentration of growth regulators as that of the induction medium resulted in shoot proliferation. An average of 12.75 shoots per explant was obtained after 30 days of culture. Individual shoots were elongated in MS medium supplemented with BAP 1.5 mg L -1 and IBA 0.5 mg L -1 . Half MS basal medium was found to be the best medium for rooting. Four to five strong healthy roots initiated in 7 days. Rooted micro shoots were successfully established in Soilrite-mix TC : garden soil (1:1) which was used as the hardening medium. The rooted explants were then gradually acclimatized and shifted to green house. Regenerated plantlets were successfully acclimatized and hardened off inside the culture room and then transferred to green house with 100 % survival rate.

In Vitro Conservation of Tinospora formanii Udayan and Pradeep-A Rare Endemic Plant from Western Ghats