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Background: AMH is a glycoprotein of the transforming growth factor-β (TGFβ) superfamily; it has as an important role in regulation of the ovarian follicle development.AMH Produced by granulosa cells of growing follicles after birth.Polycystic ovary syndrome (PCOS) is the most common cause of menstrual dysfunction, infertility, and hyperandrogenism, and itsprevalence is about 5-10% in female population of reproductive-age women. Follicular fluid (FF) surrounding the oocyte may play an important role in determining oocyte quality and the future potential to achieve fertilization and development of embryo and it provides a very good environment for the development of the oocytes. Objective: Compare the level of follicular fluid of anti-mellerian hormone (AMH) in women with PCOS and normal ovulatory women that undergoing intra cytoplasmic sperm injection (ICSI) techniques. And in prediction of clinical outcome (pregnancy) of intra cytoplasmic sperm injection (ICSI) in women with PCOS by measuring the cut-off value of FF of AMH levels. Subjects and Methods: This case-control study was carried out at College of Medicine, University of Baghdad and at Kamal Al-Samarai Hospital, Baghdad, Iraq, during the period from December 2014 to February 2016. The study involved 23 women with PCOS and 22 healthy women (male factor). Follicular fluid samples were collected from all women on day of oocyte pick up by U/S guidedtransvaginal technique and used for measurements of the concentrations of AMH. ELISA technique was used for hormone estimations. Results: The results of this study revealed that follicular fluid (FF) concentrations of AMH were significantly increased in PCOS women when compared to healthy women (P= 0.034 ). The cut-off values of FF of AMH levels for prediction of pregnancy of ICSI in PCOS women were found to be 110.17 ng/ml. In healthy women with normal ovulatory cycle, the cut-off values of FF levels of AMH were found to be 85.46 ng/ml. Conclusion: This study revealed the clinical importance of the measurements of FF levels of AMH in prediction of cases of PCOS in women’s undergoing ICSI techniques.

iosr journal of biotechnology and biochemistry

Comparison Between Follicular Antimüllerianhormonelevels Between Women’s Withpolycystic Ovarian Syndrome And Normal Ovulatory Women Undergoing Intracytoplasmic Sperm Injection (ICSI) Techniques.