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Eggplant (Solanum melongena) is recognized as one of the most important members of the Solanaceae family. Knowledge of brinjal genome organization is rather limited compared to other Solanaceous crops, especially tomato and potato. It is essential to develop an efficient method to build the fingerprinting database of commercial brinjal hybrids and their parental lines for seed purity analysis. Use of morphological differences, between true hybrids and off types in grow out test (GOT) for genetic purity analysis, are not always apparent and cannot be recognised easily. Further, morphological traits are costly, tedious to score and environment sensitive. Alternatively, it is suggested that recent breakthrough in molecular markers can be employed in genetic purity analysis. The objective of this investigation were to fingerprint commercial brinjal hybrid and their parental lines using SSR molecular markers to develop an efficient fingerprinting method for seed purity analysis in agriculture production. Brinjal Fı hybrid 555×RJ and it’s parents 555 as female and RJ as male were studied for identification and genetic purity testing with high resolution melt analysis and agarose gel electrophoresis. There are 48 brinjal microsatellite loci i.e. SSR primers were used for the analysis. Microsatellite loci, P22 were found to be heteroallelic for parents. P22 identified female specific 180bp repeat and male specific 190bp repeat. However, hybrid (555×RJ) exhibited the alleles of both parents, confirming the heterozygosity of the hybrid with the presence of two bands at 180bp and 190bp. High resolution melt analysis shows three different melting curves for male, female and hybrid. The result suggested that agarose gel electrophoresis and high resolution melting curve analysis could be used as potential, efficient and valuable methods for genetic purity analysis of brinjal hybrid and parental lines, and high resolution melting curve analysis should be given priority compared with agarose gel electrophoresis for its high accuracy and high

iosr journal of biotechnology and biochemistry

The Application of High Resolution Melting in the Analysis of Simple Sequence Repeat Markers for Fingerprinting of Brinjal (Solanum melongena L.) Hybrid and its Parental Lines