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Occurrence of somaclonal variants is a common and persistent feature of TC industry. The somaclonal variants are important source of genetic variation in asexually propagated plant species and thus are considered as a potential mean for development of new crop variety. Present study showed identification of DNA markers (SSR) linked to off-type traits in micropropagated banana. For which three category of off-type plants (O1,O2 & O3) were characterized and used for DNA isolation. A total of 150 SSR and ISSR markers were screened, all one hundred fifty SSR markers showed monomorphic banding pattern. Out of these markers only nine of them were screened and selected for CAPS marking, out of which only one marker MaCIR112 showed slight polymorphism. The PCR product of the same marker was then digested using restriction enzyme, and only Pst I showed polymorphic bands between off-types and normal plants.Digested product of MaCIR112 and some undigested product of off-types were then sequenced and SCAR marker was developed. Newly developed marker was then validated further for confirmation of polymorphic banding pattern between off-type & normal plants. Abbreviation: CTAB-Hexadecyl trimethyl ammonium Bromide; ISSR-inter-simple sequence repeat; SSR-simple sequence repeat, TC – Tissue Culture.

Identification of SSR & ISSR Markers Associated to Off-Type Somaclonal Variants in Micro-Propagated Banana