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The efficacy of different extracts of neem leaf on soil borne fungi M. phaseolina and chemical characterization of the neem leaf extracts were studied in vitro on the culture medium. The growth of M. phaseolina was inhibited significantly and controlled with both alcoholic and water extract of all ages and of the concentrations used. The alcoholic extracts of neem leaf was most effective (62.1 and 64.8%) in comparison to aqueous extract (40.0 and 61.1%) for retarding the growth of M. phaseolina. The identification of active component was done by comparing the retention time (Rt ) and chromatographic peaks of sample of Azadirachta indica leaves with their active component Azadirachtin. The gas chromatography fingerprint profile of the sample of Azadirachta indica leaves showed major peaks at the retention time of 9.82 min whereas, the pure standard of azadirachtin showed major peaks at the retention time 9.74 min. Kewords: Antifungal, Azadirachtin, Gas chromatography, Macrophomina phaseolina, Neem.

Antifungal activities of neem leaf extracts on the growth of Macrophomina phaseolina in vitro and its chemical characterization