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Clarification of the molasses is an important step in the sugar industry. The waste effluent from the lime phospho-floatation method of clarifying molasses is subjected to a feasibility study as feedstock for bacterial production of poly-3-hydroxybutyrate P(3-HB) a well known bioplastic. A chemical analysis of the waste effluent has been done to ascertain the total assimilable carbon content in terms of glucose and sucrose, along with the micronutrients like calcium, phosphates and ascorbic acid (vitamin C). Different proportions of waste effluents and inorganic mineral salts are tested in terms of P(3-HB) accumulation by the organism BP/SU1 of Staphylococcus epidermidis, origin. The production of P(3-HB) is optimized against the best utilization of waste effluent and biomass generation. The optimal proportion of waste effluent and inorganic mineral salts is upgraded to 1.5 litre bioreactor scale and the purity of the product thus obtained is confirmed.

Poly-β-hydroxybutyrate (Bio-plastic) production utilizing Waste Effluent of a Sugar Industry