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The present study was undertaken to evaluate the effect in vitro induction of sub-acute oral dose of imidacloprid and ameliorating role of tea and Vit.C on oxidative stress and osmotic fragility of albino wistar rats. On the basis of imidacloprid having LD50 450mg/kg body weight in rats, which exert oxidative stress on mammalian system and is moderately toxic via oral route. We observed a significant alteration in osmotic fragility of rat erythrocytes upon imidacloprid treatment shown by mean erythrocyte osmotic fragility at relatively higher NaCl concentration of (0.45%) as compared to mean erythrocyte fragility(MEF) at 0.40%,0.41% 42%,0.32% and 0.30% NaCl in control, Vit. C, tea, IP+ tea, IP + Vit.C treated groups, respectively. The results suggest that imidacloprid induced toxicity exerts OF in erythrocytes of rats and pretreatment with Vit. C and tea can mitigate to some extent the toxic effects.

Imidacloprid induced osmotic fragility in erythrocytes of rats: Protective role of vit.C and tea