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Fagopyrum esculentum Moench (Buckwheat) is a nutritionally important pseudocereal because of its high protein content and balanced essential amino acid profile. In the present study, the seed sample and seed protein concentrates (SPCs) of Buckwheat extracted by different procedures were tested for their antioxidant activity by DPPH free radical assay. The maximum free radical inhibition by crude seed extract at concentration 100 µg/ml was found 78%. However, the Lactobacillus fermented SPC shown 73% inhibition. A comparative antioxidant capacity of SPCs were also studied and the maximum antioxidant activity (73.10%) was recorded in Lactobacillus fermented SPC followed by coagulation at pH 4.5 (68.35%) while minimum (62.54%) was found in heat coagulated SPC. The study indicates that crude seed extract showed higher radical scavenging activity than SPC. On the other hand, the Lactobacillus fermentation of SPC raises the antioxidant activity while this property decreases at high temperature.

Free Radical scavenging activity of Fagopyrum esculentum Moench. Seed and seed protein concentrat esextracted by different procedures