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Dal Lake a Sub Himalyan urban Lake is one of the most beautiful lakes of India and second largest in state of Jammu & Kashmir. The surrounding area of Dal lake and its floating gardens form the most fertile area where a variety of vegetables are grown and extensively consumed by the inhabitants of Dal Lake. An indiscriminate use of wide variety of pesticides have been witnessed to combat pest and increase the yield. The present study was planned to assess the burden of chronic exposure to pesticides by estimation of pesticide residual levels in human serum samples taken randomly from the inhabitants of Dal Lake hamlets. Exposure of humans to hazardous chemicals occurs directly in the fields and indirectly due to consumption of contaminated diet or by inhalation or by dermal contact.The study was conducted from 2008 – 2010 in which a total of 600 blood samples taken from three population groups designated as PG-1 ,PG-2 (Study groups) and PG-3 (Control group). Blood samples were analysed for seven commonly used pesticides viz. Butachlor, -HCH, chlorpyrifos, Hexaconazole, Endosulfan 1,Endosulfan 2 and Dichlorovos. Detection and quantification of pesticide residues was performed by Gas Chromatography-Mass spectrometer (Thermo Finnigan Polaris Q type) equipped with Ni Electron Capture Detector. Out of seven pesticides analysed only chlorpyrifos was detected in all the three population groups. Mean concentration of chlorpyrifos in study groups (PG-1 & PG-2) was 0.5194 ± 0.6456 ng/μl and in control group (PG-3) was 0.0008 ± 0.0009 ng/μl. An ANOVA (Analysis of variance) was carried out and a difference was found highly significant in mean concentration of chlorpyrifos between PG-1, PG-2 and PG-3 with F value of 33.39 and P value of 0.000.Tuckey HSD (Honest Significant Difference) test reveals that PG-1 & PG-2 did not differ (P value 0.300) in their mean concentration. PG-3 differs significantly with PG-1 & PG-2 (P value 0.000). This reveals that mean level of pesticide concentration is higher in study population than control group. The findings suggest that chronic low dose exposure to pesticides either directly or indirectly can be a major contributor for presence of pesticide residual levels in human blood.

Pesticide Residues In Blood Serum Samples from Inhabitants of “Dal Lake” hamlets „in Jammu & Kashmir, India (2008-2010).