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The objective of this research is to see the comparison of protein characterization from three kinds of Indonesia edible bird nest as they have not been researched before. This research would give us overall picture on protein characteristics of the three Indonesian edible bird's nests, so it could show their activeness compared to the white edible bird nest. This research was a laboratory research with three treatments and five parameters. Treatments in this research was conducted on three kinds of edible bird nest, the white edible bird nest (Collocaliafuciphaga), the black edible bird nest (Collocalia maxima) and the swallow edible bird nest (Collocalialinchi). Parameters measured in this research were protein content, glycoprotein content, SDS- PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel) CBB (Coomassie brilliant blue) staining, SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel) glycoprotein staining and LC-MS/MS(Liquid Chromatography- mass Spectroscopy/mass spectroscopy) analysis. It was an explorative research. The method used was trial method with randomized complete design (protein and glycoprotein content) and descriptive method. The analyses results on protein and glycoprotein content from the three Indonesian edible bird nests were different and presumably due to their different kinds of feed. Protein characterization using SDS-PAGE CBB staining showed protein band high intensity on various molecular weights. They are 140.8; 64.8; 21.2 kDa for the white edible bird nest, 27.1; 21.2; 15.2 kDa for the black edible bird nest, and 140.8; 116; 36.2 kDa for the swallow edible bird nest. Glycoprotein characterization using SDS-PAGE glycoprotein staining showed different results; 140.8 and 64.8 kDa for the white edible bird nest; 140.8 and 116.0 kDa for the swallow edible bird nest; 28.4 and 105.3 kDa for the black edible bird nest. Analysis results using LC-MS/MS showed the presence of glycoprotein in the white edible bird nest but none was found in the black and the swallow edible bird nest.

iosr journal of agriculture and veterinary science

Protein Characterization of Extracted Water from Three Kinds of Edible Bird Nest Using SDS-PAGE CBB Staining and SDSPAGE Glycoprotein Staining and LC-MS/MS Analyses