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The investigation was aimed to evaluate the bulls for breeding soundness by assessing their fresh semen. The study was performed in District AI center, Mymensingh and AI center,BAU, Mymensingh. Four different bulls of cross breed (Holstein-Friesian × Zebu, Sahiwal × Zebu, Sindhi × Zebu and Red Chittagong Bull) were examined physically with a special emphasis to the palpation of the scrotum and testicles and rectal palpation of the pelvic genitalia were carried out once every two weeks. The sexual behavior particularly the libido was observed at service. A total of 107 ejaculates were collected and evaluated with respect to ejaculate volume, mass activity, sperm motility, concentration of sperm, pH, percentages of live and dead spermatozoa from 1 May to 31 October, 2013. The bulls used in this study were regarded as clinically normal. During the study, mean value of semen ejaculate volume, sperm motility, mass activity, sperm concentration, percentage of live and dead sperm and pH were 5.81± 0.16ml, 66.64± 0.50%, 3.59± 0.05, 1115.97± 16.08 million/mm 3 , 77.62 ± 0.63 %, 15% to 45%,6.48± 0.01 respectively. The mean fertility rate (65.37± 0.31%) of the bulls was also recorded. The mean values of semen ejaculate volume, sperm motility, mass activity, sperm concentration, percentage of live sperm and dead sperm, pH and fertility varied significantly (p<0.01) among breeds. Significantly (P<0.01) the highest volume per ejaculation, sperm concentration, mass activity were found in Holstein cross and lowest in Red Chittagong breeds of bulls. Significantly (P<0.05) the highest live spermatozoa percentage, pH, fertility rate were found in Holstein - Friesian × Zebu and lowest in Red Chittagong breeds of bulls. It was concluded that the Holstein - Friesian × Zebu crossbred bull produced better quality semen in comparison to other breed. So, it can be suggested that, Holstein - Friesian × Zebu crossbred bull could be suitable as breeding bull to be used for AI.

Evaluation of semen for breeding soundness of four different breeds of bull used for artificial insemination