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The investigation was conducted with the aim of artificial induction of mutation by sodium azide (SA) to overcome the limitation of variability in black gram and to induce specific improvement without disturbing their better attributes. Seeds of two varieties of urdbean viz. KU92-1 and KU300 were treated with SA of different concentrations (0.5%,1.0%,1.5%) to determine their mutagenic sensitivity in terms of seed germination, seedling height, number of branches/plant, number of leaves/plant, root length, root lets number/plant etc. One part of the mutagen treated seeds as well as the control of both the varieties were sown in plastic petri dishes and grown under room temperature and other part was planted in the experimental field following randomized block design (RBD) layout to raise first mutant generation (M1). With increase in the dose of SA, germination percentage, seedling growth and number of leaves/plant etc. were decreased. Both the parental varieties differed significantly between themselves for both percentage seed germination, seedling height and most of the characters at seedling stage for SA treatments. KU300 were highly sensitive and did not germinate in all treatments under field conditions. Variances among treatments were also significantly high.

Mutagenic Sensitivity in Early Generation in Black Gram (Vigna mungo L. Hepper)