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Nanobiosensors represents a recent sensitive accurate diagnostic tool than convenient diagnosis of microbial pathogen. Methicillin-resistant Staphylococcus aureus (MRSA) infection has increased steadily worldwide. In the present study a DNA nanobiosensor for mecA gene of MRSA detection was designed. Target gene detection was done by cross linking protocol (two probes method) using two different oligonucleotides probes that conjugated to two sets of gold nanoparticles (GNPs). The current method depended on optical properties of functionalized gold nanoparticles. Statistical analysis for the results showed specificity of 100 % and sensitivity of 90.9 % of the used technique in correlation with culture and standard coagulase tests as well as mecA PCR results. In conclusion: The presented mecA biosensor determined the correct genotype of MRSA strains in a colorimetric simple rapid procedure.

iosr journal of agriculture and veterinary science

Preparation of mecA Biosensor Based On Gold Nanoparticles to Determine Methicillin Resistant Staphylococcus Aureus (MRSA) Strains from Human and Animals