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This experiment was carried out to investigate the effects of various surface sterilants and antioxidants during in vitro propagation of guava (Psidium guajava L.). Different sterilants and exposure time significantly affected survival in shoot tip explants. Maximum survival response (43.3%) was observed when shoot tips were exposed to 2% NaOCl followed by 0.05 % HgCl2 and 4% CaOCl with 36.7 and 33.3 % survival respectively. Similarly 5 and 10 minutes exposure times were statistically at par with each other. The interaction between different sterilants and exposure time was non significant. As compared to soot tips explants, the effect of different sterilants on the survival of nodal explants was also significant. The highest survival response of 31.7% was shown by 0.05% HgCl2 followed by 4% CaOCl and 2% NaOCl (29.2 and 26.7%) respectively. Similarly the response of different exposure times (5 and 10 minutes) in case of nodal explants was non significant. Among all anti oxidants and incubation periods applied, dark incubation of cultures for 24 hours was effective which eliminated (34.3%) browning followed by dip of explants in 75:50 mg l -1 citric acid and ascorbic acid (31.8%) while control was inferior to all and gave 11.7% browning elimination. Similarly the effect of antioxidants on type of explants (shoot tip and nodal explants) was also significant. The highest response of 37.8 % was recorded in shoot tip explants while in nodal explants it was 14.5% only. The inter action between antioxidants and explants types was also significant. In over all, the highest browning elimination of 55% was recorded in shoot tip explants when cultures were kept in dark for 24 hours. Whereas the most inferior results were shown by control treatments where only 6.7 and 16.7% browning was eliminated in tips and nodal explants.

Effect of exposure time and incubation period of various sterilants and antioxidants on the in vitro morphogenesis of guava explants