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The study was conducted to screen the milk samples for drug residual contamination marketed at the vicinities of Hyderabad, Mirpurkhas, Sukkur, Larkana, and Karachi divisions of Sindh province. Out of total 400 samples, 49.75% were found positive for antibiotic residues, and over residual positive samples the highest percent was in the vicinity of Karachi followed by Hyderabad, Sukkur, Larkana and Mirpurkhas. The overall mean zone size was measured as 14.27±0.26mm in milk marketed at different areas of Sindh. Milk samples positive to antimicrobial drugs were further screened through Twin Sensor BT KIT020, and found that β-lactam was highest at Larkana followed by Hyderabad, Karachi, Mirpurkhas and Sukkur. The residual limit of Tetracycline was higher in samples obtained from Hyderabad, relatively similar percent was found in milk samples from Mirpurkhas and Larkana and least in samples of Sukkur. The overall residual contamination in milk was higher (38.29%) for β-lactam than that of Tetracycline (21.14%) group. It could be concluded that indiscriminate pattern or misuse of antibiotics at various stages of milk processing may have shown the significant influence and the residual level in milk reached at the peak point.

Detection of antimicrobial drug residues in milk marketed at different areas of Sindh province