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The study was conducted to analyze the effect of bacterial cell wall peptidoglycan on the induction of toll-like receptor2 in peripheral blood mononuclear cells (PBMCs). The cultures were stimulated with B. subtilis cell wall peptidoglycan (sPGN) at final doses of 10, 20, 30, 40 and 50 µg/ml and incubated for 3 h, 12 h, 24 h and 36 h time periods, for all the treatments. We observed that sPGN not only activates TLR2 expression in PBMCs, in a dose-and-time dependent manner, but also induces tolerance to further expression, beyond a certain concentration of the antigen, irrespective of the incubation interval. In our study, PBMCs became refractory to further stimulation by sPGN beyond 30μg/ml dose across all the incubation intervals, ranging from 3h to 36h. The highest TLR2 induction across all doses of the antigen was observed at 24h incubation interval, suggesting probably the optimum physiological response to the antigen. We also cloned and sequenced TLR2 expressed sequence tag from buffalo PBMCs and submitted the sequence to GenBank. Keywords: Buffalo, Peripheral blood mononuclear cells, peptidoglycan, TLR2, tolerance.

iosr journal of agriculture and veterinary science

Expression Analysis of Toll-Like Receptor2 in Bubaline Peripheral Blood Mononuclear Cells upon Stimulation by Bacillus Subtilis Cell Wall Peptidoglycan